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In order to control the invasion of harmful aquatic organisms and pathogens in ballast water effectively, the International Maritime Organization (IMO) has developed the International Convention and Management of Ship’s Ballast Water and Sediments in 2004. The convention came into force since 2009 and requires that new ships must install ballast water treatment systems and this requirement is also suitable for the existing ships. Meanwhile, the convention had set regulation D-2 which regulated the category and the quantity of the surviving organisms in treated ballast water.